Kyoko Aono (青野 響子 Aono Kyōko?)
Voiced by: Sachiko Kojima
Kyoko Aono is Tsukune's tomboyish 17-year-old cousin with a stubborn streak. She is overprotective of Tsukune, whom she refers to as "Tsukkie", and makes her appearance on the second day of the school festival while searching for Yōkai Academy. Tsukune and his friends attempt to keep her from finding out about the academy's nature and its students, as well as prevent the authorities from discovering she is human. Kyoko manages to enter the yōkai world to deliver a package that contains the Lilith Mirror (リリスの鏡 Ririsu no Kagami?). Housing a fairy named Lilith (voiced by Maria Kawamura), it claims it grants the owner any wish they desire, but at the cost of their soul. Later, Kyoko is led to believe Tsukune is involved with the Yakuza.
In the anime, she was briefly mentioned in the first series, but makes a full appearance in the second season when she accidentally obtains the Lilith Mirror from a run-in with Kokoa while she was delivering rice cakes for Tsukune (both parcels have the same wrapping), with Kokoa originally planning to use the mirror to bring back Inner Moka.
Kasumi Aono (青野 かすみ Aono Kasumi?) and Koji Aono (青野 光司 Aono Kōji?)
Voiced by: Haruha Terada (Kasumi)
Tsukune's parents and Kyoko's aunt and uncle, respectively.
Doppleganger
A thief who specializes in transforming into a person close to his targets then killing them. When he transforms into a monster, he gains all of their abilities, but also all of their weaknesses. To compensate for his weak powers, he has learned all basic marital arts. His minions are a Minotaur and a spider-man. Despite his ruthlessness, he is gynophobic.
Issa Shuzen (朱染 一茶 Shuzen Īssa?)
Voiced by: Katsuji Mori
Issa Shuzen is Moka's father and the head of the Shuzen Family, which is well-known and respected as far as China for its reputation of dealing with "problems" in the underground. It is also the only location in Japan that houses vampires. Upon Akua's arrival to the mansion from China, he orders her and Kahlua to kill each other, so that he may test his eldest daughter's strength. He stops the fight before either one becomes badly injured, however, having measured her ability to his satisfaction.
Issa makes a full appearance in Rosario + Vampire Capu2 and is the third Dark Lord. In this adaptation, he created Moka's rosario and the one used for the school barrier. His contempt for humans knows no bounds and according to Komori, his powers exceed that of a standard S-class yōkai, able to throw anyone with just a wave of his hand.
Marin Kawamoto (川本 まりん Kawamoto Marin?)
Marin is an owner of a snack bar and a mother figure to Sun. She knows about yōkai since her husband was killed by Kanade 4 years ago.
Touhou Fuhai (東方不敗 Tōhō Fuhai?)
Touhou Fuhai is the second Dark Lord introduced in chapter 27 of the manga, and, according to Ling-Ling, responsible for teaching Mikogami his sealing techniques. He is also the founder of the Wong Crime Family in China. It is revealed that he's an otaku and cosplay fanatic when he tricks Inner Moka into dressing up as Lum Invader from Urusei Yatsura before repairing her rosary. He claims that since he's gotten older, women aren't drawn to him anymore, and in order to cope, he's turned to manga. He further displays this when he shouts that "2D girls last forever", and they would never betray him. He is also an old friend of Akasha Bloodriver, Moka's mother.
Fei-Fong Wong (黄 飛鴻; katakana: ウォン フェイフォン Won Feifon?) and Ten-Ten Wong (黄 恬恬; katakana: ウォン テンテン Won Tenten?)
Ling-Ling's and Fong-Fong's parents. Fei-Fong is head of the Wong Crime family and the great-grandson of Touhou Fuhai who fell in love with Ten-Ten, the daughter of the Miu family and a master martial artist. Both families are intense rivals, but were able to put their feud aside to allow them to get married. Fong-Fong describes his parents as Romeo and Juliet from "Romeo and Juliet" and states that Ten-Ten is stronger than Fei-Fong.
Akasha Bloodriver (アカーシャ・ブラッドリバー Akāsha Buraddoribā?)
Akasha Bloodriver is Moka's mother, whose appearance is exactly like "Outer" Moka's. Touhou Fuhai, the second Dark Lord, is a good friend of Akasha and describes her as a strong yet sad woman who carried a heavy burden. Despite being kind and gentle, Akasha is one of the true ancestors of the Vampire race and managed to defeat the most powerful Vampire Lord at her time, thus making her a Dark Lord and the leader of the three. However, when she defeated the Vampire Lord Alucard (アルカード Arukādo?), her blood was synchronized with his blood, meaning that if Akasha unleashed her full power, it could cause Alucard to awaken once more. This caused even more trouble for her when she gave birth to Moka. Because Moka was on the verge of death when she was born, Akasha injected much of her blood into Moka to keep her alive. Therefore, Moka also possesses the "Shinso Blood" and could awaken Alucard from his slumber. After Moka discovered the corpse of the Vampire Lord, Akasha forced Moka to leave her home in order to protect her. She destroyed all of her photos and diaries, leaving behind no connections except the rosary seal used to protect Moka. She is the orignal user of the quote "Know your place!" (身の程を知れ!?), a quote that was later taken up by her daughter. Her last name may be a pun to the word "Blood bank".